bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis [ChIP-seq]

To reveal genome-wide direct targets of AtbZIP17, we carried out Chromatin immuno-precipitation coupled with high-throughput sequencing (ChIP-seq) experiments with heat-stressed MYC-bZIP17 expressing seedling plants. For ChIP-seq, 13-day-old 35Spro::MYC-bZIP17 seedlings grown at 22°C were placed at 42°C for 2 h. There were 4 samples in ChIP-seq analysis, 13-day-old 35Spro::MYC-bZIP17 seedlings grown at 22°C were placed at 42°C for 2 h, lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The ChIP-DNA and INPUT DNA libraries were constructed and sequenced by Genergy Bio (Shanghai, China) with Illumina Novaseq 6000.