Nanostring expression assay for positionally conserved lncRNAs

This dataset contains expression data for positionally conserved lncRNAs measure using the Nanostring assay. Sheet A: Annotation of probes used in the assay. B: List of samples tested in the assay. C: Normalised expression of tested human and mouse pcRNAs and associated coding genes.MethodsWe designed probes to detect 50 pairs of pcRNAs and corresponding coding genes in human and mouse. The probes were designed according to the Nanostring guidelines and to maximize their specificity and included 9 house-keeping genes for normalization (ALAS1, B2M, CLTC, GAPDH, GUSB, HPRT, PGK1, TDB, TUBB). The raw count data were first normalized by Nanostring Technologies with the nSolver software using a two-step protocol. First, data were normalized to internal positive controls, then to the geometric mean of house-keeping genes. The normalised data was then imported into R for further analysis.