Overview of EAV nsp1 pseudorevertants described in this study.
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aVirus clones were isolated from plaque assays of culture supernatants harvested between 20 h and 24 h post transfection. The number of clones containing each mutation is indicated in brackets.
bNumbers indicate the start coordinate of the codon in the EAV genome.
cPseudorevertants were reconstructed in the wild-type EAV background, and infectious progeny titers were determined by plaque assays of culture supernatants harvested at 24 h post-transfection. The average titers of three independent experiments are shown.
dNA, not applicable.
eA silent mutation changing the original alanine codon from GCG to GCC was introduced as a marker mutation upon construction of the ZCH mutant.
创建时间：
2013-02-21
