RNA-seq
收藏NIAID Data Ecosystem2026-03-13 收录
下载链接：
https://www.omicsdi.org/dataset/node/OEX00011132
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资源简介：
Total RNA was extracted and purified from fresh frozen tissues using the Whole RNA extraction kit (TIAN GEN). RNA integrity was measured on an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with enough amount and good quality were used to prepare the transcriptome library. The cDNA synthesis, end-repair, A-base addition, and ligation of the Illumina index adapters were performed according to QuarPrep RNA Library Kit (DynastyGene Biotechnologies). Library quality was measured on an Agilent 2100 Bioanalyzer for product size and concentration. Paired-end libraries were sequenced by an Illumina NovaSeq 6000 System, with a sequence coverage of 139 million paired reads.
创建时间：
2021-10-16
