Gene expression analysis of RACB-transgenic barley plant lines in interaction with Blumeria graminis f.sp. hordei. Hordeum vulgare

To analyze RACB- and Bgh (Blumeria graminis f.sp. hordei)-dependently expressed genes we used wild type and transgenic barley misexpressing the susceptibility factor RACB Overall design: Gene expression was analyzed at 12 hours post infection (hpi) and 32 hpi in untreated and Bgh-treated samples in three plant lines (wild type (WT); expressing constitutively activated (CA) RACB; RNAi RACB). In total 4 biological replicats were performed.