Interferon Regulatory Factor 5 represses mitochondrial matrix protein GHITM to limit macrophage oxidative capacity in early response to obesity [bmdm]

To investigate the involvement of IRF5 in macrophages during obesity-related inflammation, we analyzed bone marrow-derived macrophages stimulated for 2 or 24 hours with Lipopolysaccharide (LPS), Palmitate or carrier (bovine serum albumin), from mice with a myeloid-specific deletion of IRF5 or their wild-type littermates. Overall design: RNA-seq analysis from bone marrow-derived macrophages stimulated for 2 or 24 hours with LPS or with Palmitate. Studies were carried out on mice with a myeloid-specific deletion of IRF5 or their wild-type littermates.