Ectopic expression of miR-181a enhances lymphoid differentiation in the ETV6/RUNX1-positive cell line and primary ALL blasts.

(A) Percentage of lentivirus-infected REH cells stained for cell-surface marker CD10 as analyzed by flow cytometry (left). The results were quantified and are presented as the average ± SD of three independent evaluations (right). (B) 181A1-LV-infected REH cells were co-stained for annexin V and CD10 and measured by flow cytometry (left). The percentage of each of CD10-positive and -negative annexin V-positive cells was quantified, and the average ± SD of three independent evaluations is shown (right). (C) Primary ALL blasts were infected with NC- or 181A1-LV. Mature miR-181a expression level in lentivirus-infected primary pre-B ALL blasts from three ETV6/RUNX1-positive patients was determined by qRT-PCR. The results are shown as average ± SD. (D) CD10 expression in CD45w+or+ CD19+ ETV6/RUNX1-positive pre-B ALL blasts was analyzed by flow cytometry. In the comparison with the infection control (NC), 181A1-LV-transduced cells derived from patient #1 and #2 both showed a decrease in CD10High cells (Patient #1: NC 10.1% / MFI 40.92, 181A1 6.95% / MFI 39.51; Patient #2: NC 40.1% / MFI 424.28, 181A1 29.5% / MFI 444.24) and a relative increase in CD10Low cells (Patient #1: NC 87.98% / MFI 165.49, 181A1 91.05% / MFI 156.68; Patient #2: NC 58.1% / MFI 1929.88, 181A1 69% / 1854.4). *P ≤ 0.05, **P ≤ 0.01 (ANOVA).