PFG controls gene expression in female gametocytes cooperatively with AP2-FG [AP2-FG_DIP]

AP2-FG is a female-specific transcription factor (TF) that plays an essential role in female gametocyte development. AP2-FG activates hundreds of genes by binding to a female-specific ten-base cis-acting element. Here, we report that in the rodent malaria parasite Plasmodium berghei, another female-specific TF designated as a partner of AP2-FG (PFG), controls gene expression in female gametocytes cooperatively with AP2-FG. Transcriptional mechanisms were analyzed in Plasmodium berghei female gametocytes. Overall design: AP2-FG_DIP The recombinant AP2 domain of AP2-FG was prepared as a GST-fused protein. This recombinant GST-fused AP2 domain of AP2-FG and genomic DNA fragments were incubated at RT for 30 min in 100 µL of binding/washing buffer. The mixture was then incubated with 50 µL glutathione sepharose resin (Cytiva) at RT with rotation. Resin was washed three times with 150 µL of binding/washing buffer, and bound protein was eluted with 10 mM glutathione solution. Input genomic DNA fragments were also sequenced as a control.