T-cell responses to a specific antigen

The goal of this study is to demonstrate the ability of a novel platform to screen, identify and characterize neoantigen-specific T-cells and their receptors by combining targeted RNA-seq data on subset of T-cell specific genes and T-cell Receptor sequencing In this study, we demonstrate the ability to use hybrid-capture based enrichment for a subset of 17 T-cell specific genes on 3' Digital Gene Expression (DGE) RNA-seq libraries. The samples processed were CD8+ T-cells enriched from blood from two independant healthy donors with each pool of CD8+ T-cells being either unstimulated (control) or stimulated with the Cytomegalovirus, Epstein-Barr Virus and Influenza Virus (CEF) control peptide pool overnight to monitor for T-cell activation.