Excitation energy transfer kinetics of trimeric, monomeric and subunit-depleted Photosystem I from Synechocystis PCC 6803

We present spectroscopic comparison of trimeric Photosystem I from the cyanobacterium Synechocystis PCC 6803 with a monomeric complex from the Δ<i>psaL</i> mutant and a “minimal” monomeric complex ΔFIJL, containing only subunits A, B, C, D, E, K and M. We have collected UV-Vis absorption spectra, circular dichroism spectra and picosecond fluorescence kinetics, at room temperature and 77 K, of isolated Photosystem I complexes and thylakoid membrane fragments of the respective strains.<br><b>File abbreviations</b>CD - circular dichroismDAES - decay-associated emission spectraFIJL - mutant lacking subunits PsaF/I/J/LpsaL - Δ<i>psaL </i>mutantPSI - Photosystem I (isolated)RT - room temperatureThyl - thylakoid membranesTRF - time-resolved fluorescence (kinetics)<br>WT - wild-type Synechocystis PCC 6803 <br>