Additional file 1 of Stem cell-derived brainstem mouse astrocytes obtain a neurotoxic phenotype in vitro upon neuroinflammation

Additional file 1: Supplementary Methods; Supplementary References. Supplementary Table 1. Media constituents for motor neuron differentiation. Supplementary Table 2. Media constituents for astrocyte differentiation. Supplementary Table 3. Media constituents for primary SVZ cell culture. Supplementary Table 4. Neuroinflammatory factors used. Supplementary Table 5. Materials used for immunocytochemistry. Supplementary Table 6. Buffers used for flow-cytometry and fluorescence-activated cell sorting. Supplementary Figure 1. Glial fibrillary acidic protein is not an optimal marker of ES-astrocyte activation induced by IL-1 α and TNF- α. Supplementary Figure 2. Primary culture validation in subventricular zone-astrocytes. Supplementary Figure 3. Contact-independent co-culture system of astrocyte-mediated neurotoxicity. Supplementary Figure 4. Immunocytochemical depiction of translationally relevant neuroinflammatory mediators.