Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14, E280A mutant in complex with the xyloglucan tetradecasaccharide XXXGXXXG

Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14, E280A mutant in complex with the xyloglucan tetradecasaccharide XXXGXXXG Descriptor: B-1,4-endoglucanase, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose Authors: Morar, M, Stogios, P.J, Xu, X, Cui, H, Di Leo, R, Yim, V, Savchenko, A. Deposit date: 2015-08-18 Release date: 2015-11-04 Last modified: 2023-09-27 Method: X-RAY DIFFRACTION (1.9 Å) Cite: Structure-Function Analysis of a Mixed-linkage beta-Glucanase/Xyloglucanase from the Key Ruminal Bacteroidetes Prevotella bryantii B14. J.Biol.Chem., 291, 2016

PbGH5A的晶体结构：该酶为来自布氏普雷沃氏菌（Prevotella bryantii）B14的糖苷水解酶家族5（glycoside hydrolase family 5, GH5）的E280A突变体，与木葡聚糖十四糖XXXGXXXG形成复合物。
样本描述：β-1,4-内切葡聚糖酶，底物分子结构为α-D-吡喃木糖-(1→6)-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-α-D-吡喃葡萄糖，α-D-吡喃木糖-(1→6)-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-[α-D-吡喃木糖-(1→6)]-β-D-吡喃葡萄糖-(1→4)-α-D-吡喃葡萄糖。
作者：Morar, M.、Stogios, P.J.、Xu, X.、Cui, H.、Di Leo, R.、Yim, V.、Savchenko, A.
提交日期：2015年8月18日
发布日期：2015年11月4日
最后修改日期：2023年9月27日
实验方法：X射线衍射（分辨率1.9 Å）
引用文献：《来自关键瘤胃拟杆菌门布氏普雷沃氏菌B14的混合连接β-葡聚糖酶/木葡聚糖酶的结构与功能分析》，《生物化学杂志（J. Biol. Chem.）》，291卷，2016年