CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators II
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https://www.ncbi.nlm.nih.gov/sra/SRP279800
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资源简介：
Cellular RNA is decorated with over 160 types of chemical modifications. Many modifications in mRNA, including m6A and m5C, have been associated with critical cellular functions under physiological and/or pathological conditions. To understand the biological functions of these modifications, it is vital to identify the regulators that could modulate the modification rate. However, a high-throughput method for unbiased screening of these regulators is so far lacking. Here, we report such a method combining pooled CRISPR screen and reporters with RNA modification readout, termed CRISPR integrated gRNA and reporter sequencing (CIGAR-seq). Overall design: mRNA-BisSeq of HAP1 cells
创建时间：
2020-12-16
