Gene copy number variation profiling by microarray. Gene copy number variation profiling by microarray

Here, we investigate focal amplification patterns in and around the BRAF locus in BRAFV600Emutant COLO205 cells that acquire resistance to the MEK inhibitor Sleumetinib / AZD6244 / ARRY-142886. We find that BRAF amplifications occur frequently in COLO205 cells during acquisition of selumetinib resistance and that BRAFamplifications pre-exist selumetinib treatment but occurs predominantly via de novo mutations Overall design: Genomic DNA extracted from parental COLO205 cells and their selumetinib-resistant dervatives were profiled for gene copy number variation using Infinium CytoSNP 850K beadchip microarrays (Illumina)