Genetic analysis of residual feed intake and its molecular pathways in coloured broiler chicken [duodenum]

Transcriptome analysis was performed in duodenum of high and low RFI chicks for finding out differentially expressing genes and molecular pathways for RFI in colored broiler chicken. The sample of 4 birds each from high and low RFI group from the whole lot of 225 broilers was taken for transcriptome analysis. The expression of genes influencing low and high feed efficiency and the pathways of these genes was studied out to examine the molecular mechanism influencing feed efficiency. In this experiment 4-5 birds at the age of 5-weeks were humanely slaughtered each organ wise in each group (high and low RFI). Each pool was divided in duplicate resulting two biological replicate in each group. Finally, two complementary RNA from each group were used for hybridization reaction. The RNAs from low FE group were used as a control for RNA from high FE group.