Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

<strong>Project: </strong>High resolution structural analysis of purified HTT samples <strong>Experiment: </strong>Analysis of dephosphorylated HTT samples by cryoEM analysis <strong>Date completed:­ </strong>2019/05/01 <strong>Rationale: </strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.