Role and function of Pten in BCR-ABL1 driven pre-B cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34829
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In order to investigate the function of Pten in ALL, we isolated bone marrow cells from Pten fl/fl mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with CRE-ERT2 or ERT2 (PMIP) and after 2 days of treatment with 4OH-Tamoxifen (0.5 micromolar) subjected to gene expression analysis. Two days after treatment with 4OH-Tamoxifen (0.5 micromolar) total RNA of Puromycin resistant cells was extracted and subjected to gene expression analysis.
创建时间:
2019-06-26



