Expression data from free fatty acid polarized bone marrow Gr1- myeloid cells

Free fatty acids accumulate in tumor microenvironment which facilitate the polarization of bone marrow derived myeloid cells into tumor associated macrophages. However, the underlying mechanism is still unclear. Here we use microarray to detail the impact of variant free fatty acids on the maturation of bone marrow derived myeloid cells. Bone marrow isolated from Balb/c mice were cultured in the presence of 40 ng/ml GM-CSF plus variant 0.2 mM stearate (SS), oleate (SO) or BSA control. On day7, Gr1- myeloid cells were purified and lysed for for RNA extraction and hybridization on Affymetrix microarrays. .