Tiffiney R. Hartman, Daniel Zinshteyn, Heather K. Schofield, Emmanuelle Nicolas, Ami Okada, Alana M. O'Reilly (2011) CIL:13754, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. CIL. Dataset
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Hedgehog (Hh, red) is transcribed in apical cells of the Drosophila wild-type germarium (CIL# 13755, 13744). In the absence of boi (boi[e] mutant), Hh protein is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745), but Hh transcription is still in apical cells (this image; boi[e]; Hh-lacZ). Hh transcription is demonstrated by the Hh-lacZ reporter and follicle cells are highlighted using anti-Fas-3 (green). Image is Supplemental Figure S3B in J Cell Biol. 2010. 191: 943-952.
刺猬基因(Hh,红色)在果蝇野生型生殖腺的顶细胞中进行转录(CIL# 13755,13744)。在boi(boi[e]突变体)缺失的情况下,Hh蛋白从顶细胞重新分布至局部毛囊干细胞微环境的外细胞空间(CIL# 13745),然而Hh的转录仍位于顶细胞(本图;boi[e];Hh-lacZ)。Hh的转录通过Hh-lacZ报告基因得以证明,而毛囊细胞则通过抗Fas-3抗体(绿色)进行标记。该图像为《细胞生物学杂志》(J Cell Biol. 2010. 191: 943-952)的补充图S3B。
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