Neutralization capacity of monoclonal antibodies in the viremic blood neutralization assay.

Mosquitoes were fed on blood meals containing DENV infected blood and 10 μg/mL of individual mAbs, except for 747(4)B7 and 753(3)C10 at 3.7 μg/mL and 5 μg/mL, respectively. DENV infected mosquitoes were detected by RT-PCR. Odds ratios were calculated using data from each mAb with the negative saline control as the reference. P values were calculated using marginal logistic regression models, adjusted for the patient’s plasma viremia. The reduction in percentage of mosquito infection, compared with the negative saline control, was calculated for each blood feeding cohort. DENV = dengue virus; PC = positive control; NC = negative control.