Metagenomic Characterization and Comparative Analysis of Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions

This study aimed to characterize and compare the metagenomic composition of saliva in denture wearers with different periodontal statuses. 24 participants, who are older than 60 years old, were recruited and grouped into denture wearers with active periodontitis, non-denture wearers with active periodontitis, denture wearers with stable periodontal health condition, and non-denture wearers with stable periodontal health condition. Saliva samples were collected and underwent DNA extraction using QIAmp Mini DNA Extraction Kit. 2bRAD metagenomic sequencing was performed to characterize the species-resolved microbial composition. Taxonomic profiles of the microbial communities were analyzed and compared between groups.