The role of AIB1D4 cistrome in early breast cancer progression

We report the global occupancy by ChIP-seq of AIB1 in MCF10A, MCF10A-D4, MCFDCIS and MCFDCIS-D4 cells. We performed ChIP and library preparation with the ActiveMotif Low Cell ChIP-seq kit. Data was aligned to hg19 with rsubread, data was sorted and de-duplicated with SAMTOOLS, and peaks were called with CSAW. Analysis was done with ChIP-seeker in R. Examination of ChIP-seq of AIB1 isoforms binding to the genome in MCFDCIS vs. MCFDCIS-D4 cell lines in triplicate, normalized to cell lysate input. And, MCF10A vs. MCF10A-D4 cell lines in triplicate, normalized to cell lysate input.