Effects of Citrinin on DNA Methylation Demonstrated in Vitro and in Silico

SH-SY5Y and HK-2 cells were exposed to citrinin (CIT) for 24 hours. Cell viability of cell lines were assessed via MTT test. 5-methylcytosine levels and expression profiles of genes involved in DNA methylation (DNMT-1, DNMT-3a, DNMT-3b, TET-1, TET-2, TET-3) were measured. CIT and the endogenous DNMT inhibitor S-adenosyl homocysteine (SAH) were docked to DNMT-1, DNMT-3a and DNMT-3b using AutoDock 4.2. pdb files of selected poses are provided.

SH-SY5Y与HK-2细胞分别暴露于缬氨霉素（CIT）中24小时。通过MTT测试评估细胞系的存活率。检测了5-甲基胞嘧啶水平以及参与DNA甲基化（DNMT-1、DNMT-3a、DNMT-3b、TET-1、TET-2、TET-3）的基因表达谱。利用AutoDock 4.2将CIT及内源性DNMT抑制剂S-腺苷高半胱氨酸（SAH）对接到DNMT-1、DNMT-3a和DNMT-3b上。提供了选定构象的pdb文件。