Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth

We sequenced whole transcriptome from attack phase (AP) Bdellovbrio bacteriovorus HD100 after incubating it for 4 hours either in a nutrient rich meium (nutrient broth, NB) or in absence of nutrients (HEPES buffer only, H) as a control. The aim is to know how the excracellular nutrients affect the gene expression pattern of AP B. bacteriovorus. We found that the nutrients induces AP B. bacteriovorus to up-regulate its transcription and translation machinery in a way that resembles what happen during the late intraperiplasmic stage of B. bacteriovorus life cycle Overall design: B. bacteriovorus genome-wide gene expression pattern was compared upon incubating it in HEPES buffer versus Nutrient broth. Both are done in triplicate.