Far red light increases maize volatile emissions in response to volatile cues from neighboring plants

Plants perceive the presence and defense status of their neighbors through light and volatile cues, but how plants integrate both stimuli is poorly understood. We investigated if and how low Red to Far red light (R:FR) ratios, indicative of shading or canopy closure, affect maize (Zea mays) responses to herbivore-induced plant volatiles (HIPVs), including the green leaf volatile (Z)-3-hexenyl acetate. We modulated light signaling and perception by using FR supplementation and a phyB1phyB2 mutant, and we determined volatile release as a response readout. To gain mechanistic insights, we examined expression of volatile biosynthesis genes, hormone accumulation, and photosynthesis. Exposure to a full blend of HIPVs or (Z)-3-hexenyl acetate induced maize volatile release. Short-term FR supplementation increased this response. In contrast, prolonged FR supplementation or constitutive phytochrome B inactivation in phyB1phyB2 plants showed the opposite response. Short-term FR supplementation en..., , , # Data from: Far-red light increases maize volatile emissions in response to volatile cues from neighboring plants

[https://doi.org/10.5061/dryad.0rxwdbs7n](https://doi.org/10.5061/dryad.0rxwdbs7n)

## Data & File overview:

1. Excel file containing 27 excel sheets corresponding to the data used in the main and supplemental figures of the manuscript:

1. **Figure 1b.** Volatiles collected and measured by PTR-TOF-MS from both emitter and receiver maize plants.

* Number of variables: 12
* Variable list:

1. Emitter = Low Red to Far Red treated plant infested with *S. littoralis* (Low R:FR infested) or left uninfested (Low R:FR non_infested)
2. Receiver = Low or high R:FR treated plant (LowR_FR and HighR_FR)
3. Iteration = volatile sampling iteration
4. Plant = Identification number for each plant that is sampled in the time series analysis.
5. Indole (signal intensity/ grams of fresh weight)
...