What controls the puppets? 20-hydroxyecdysone may not directly drive spider manipulation by wasps in Leucauge spiders

Data associated with the manuscript:“What controls the puppets? 20-hydroxyecdysone may not directly drive spider manipulation by wasps in Leucauge spiders.”

Files

This repository contains two data files:

Data_Leucauge_volupis_Webs.xlsxAnalysis_20E_Leucauge_volupis.xlsx

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1. WEB STRUCTURE DATASET

The file Data_Leucauge_volupis_Webs.xlsx contains data related to the analysis of different web types built by Leucauge volupis spiders.

Variables

id: Identifier of each observation (replicate; one web per entry) (categorical)

web_type: Type of web

(levels: normal, molting, cocoon) (categorical)

spider_age: Developmental stage (levels: adult, juvenile/subadult) (categorical)

spider_sex: Sex of the individual (levels: female, male) (categorical)

spirals: Number of spiral loops, counted along standardized radii (integer)

radii: Number of structural radii converging to the hub (integer)

reinforced_radii: Number of radii reinforced with additional silk (integer)

tangle_thread: Presence and configuration of barrier threads (levels: absent, upper, lower, both) (categorical)

opening_hub: Hub condition (center of the web) (levels: open, closed) (categorical, binary)

hub_spirals: Number of spirals located near the hub region (integer)

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2. ECDYSTEROID ANALYSIS DATASET

The file Analysis_20E_Leucauge_volupis.xlsx contains data on ecdysteroid concentrations in parasitized and non-parasitized spiders.

SHEETS: 20E ion 371 and 20E ion 445

Trat: Treatment group - Parasitized (without modified behavior), Parasitized (with modified behavior), After molting, Non-parasitized (in character/factor)

Rep: Individual identifier (in character/factor)

Conc: Hormone concentration in pg/mg (numerical, counts)

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SHEET: General results 

These sheets contain aggregated results from the full analytical procedure.

Variables

Replicate: Sample identifier (unique code per individual) (categorical)

Product_Mz: Mass-to-charge ratio (m/z) of the detected product ion (e.g., 371 or 445) (numeric)

Area: Chromatographic peak area associated with the detected ion (numeric)

pg/1000ul: Estimated hormone quantity (pg) standardized to 1000 µL, based on calibration curves (continuous)

Quantity pg em 400ul: Absolute hormone quantity (pg) in the extracted sample volume (400 µL) (continuous)

Biomass (mg): Body mass of the spider (mg) (continuous)

Biomass (pg/mg of the spider): Final normalized hormone concentration (pg/mg), calculated as: hormone quantity (pg) divided by body mass (mg) (continuous)

Group (implicit: NP, PA_MB, PA_NB, E, L3, L2):NP: non-parasitizedPA_MB: parasitized without behavioral modificationPA_NB: parasitized with behavioral modificationE: post-molting individualsL3, L2: developmental stages (instars)

MEAN_1000: Mean hormone quantity (pg) normalized to 1000 µL (continuous)

MEAN_400: Mean hormone quantity (pg) in 400 µL extract (continuous)

SD_1000: Standard deviation of hormone quantity (pg) normalized to 1000 µL (continuous)

SD_400: Standard deviation of hormone quantity (pg) in 400 µL extract (continuous)

MEAN_CONC: Mean normalized hormone concentration (pg/mg) (continuous)

SD_CONC: Standard deviation of normalized hormone concentration (pg/mg) (continuous)

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SHEET: Summary (merge between the 20E ion 371 and 20E ion 445 sheets)Trat: Treatment group - Parasitized (without modified behavior), Parasitized (with modified behavior), After molting, Non-parasitized (in character/factor)Ion: The type of ionRep: Individual identifier (in character/factor)Conc: Hormone concentration in pg/mg (numerical, counts)

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NotesAll variables are described using consistent terminology to facilitate reuse.

Missing values (if present) are indicated directly in the dataset files.

For methodological details, refer to the main manuscript.