Real-time quantitative PCR analysis of Lentinula edodes mycelium face on different stress treatments.

L. edodes strain w1 was cultured at 25°C in MYG medium (containing 1% malt extract, 0.1% peptone, 0.1% yeast extract, and 2% glucose). Fresh mycelium blocks were cultured in a 9cm petri dish containing MYG medium. After 8-day dark cul- ture, and they were divided into different treatment groups (group A,B,C,D,E). Group A was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then treated at 37 °C for 30 min. 3. Group B was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then treated at 4 °C for 60 min. Group C was treated with 8mm-diameter L. edodes mycelium block on MYG medium for seven days and then under 3500 lx light for 1 h. In group D, 8mm-diameter fresh L. edodes mycelium block were inoculated into MYG medium containing cadmium ions. In group E, 8mm-diameter L. edodes mycelium block was inoculated at 10 mm from the edge of the petri dish, and cultured at 25 ° C for 7 days. Afterwards, an activated 8-mm diameter tricho- derma mycelium block was inoculated at 1 cm from the edge of the petri dish at one end of the petri dish diameter (corresponding to the other end where L. edodes mycelium block was inoculated) qPCR gene expression profiling. Lentinula edodes mycelium were used and treated separately as indicated in the summary.