Chlamydomonas cilia proteins

Axonemal dyneins are highly complex molecular motors that power ciliary motility. These multi-subunit enzymes are assembled at dedicated sites within the cytoplasm. At least nineteen cytosolic factors are specifically needed for the generation of dynein holoenzymes and/or their trafficking to the growing cilium. Many proteins are subject to N-terminal processing and acetylation which can generate degrons subject to the AcN-end rule and alter N-terminal electrostatics, generate new binding interfaces, and affect subunit stoichiometry through targeted degradation. Here we have used mass spectrometry of cilia samples and electrophoretically purified dynein heavy chains from Chlamydomonas to define their N-terminal processing; we also detail the N-terminal acetylase complexes present in this organism. We identify four classes of dynein heavy chain based on their processing pathways by two distinct acetylases one of which is dependent on methionine aminopeptidase activity. In addition, we fi..., These data were generated from whole cilia and electrophoretically purified dynein heavy chains digested with trypsin or Asp-N using an Orbitrap Eclipse mass spectrometer and positive mode electrospray ionization. Data were searched against the Chlamydomonas v.6.1 proteome. , , # Chlamydomonas cilia proteins

[https://doi.org/10.5061/dryad.mw6m90635](https://doi.org/10.5061/dryad.mw6m90635)

These are scaffold files that contain the proteomic data for whole cilia and electrophoretically purified axonemal dynein heavy chains from Chlamydomonas.

## Description of the data and file structure

Four scaffold files are uploaded.

1. \"Whole Cilia\" - Chlamydomonas cilia digested with trypsin or endoproteinase AspN.
2. \"Dynein Heavy Chains\" - Electrophoretically purified wildtype Chlamydomonas axonemal dynein heavy chains digested with trypsin.
3. \"Dynein Heavy Chains 2\" - Electrophoretically purified wildtype Chlamydomonas axonemal dynein heavy chains digested with trypsin.
4. \"AspN-digested Dynein Heavy Chains: - Electrophoretically purified wildtype and oda2 Chlamydomonas axonemal dynein heavy chains digested with endoproteinase AspN.