Comparative analysis of the different Streptomyces bingchenggensis strains by RNA-seq

Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins are commercially insecticidal and acaricidal antibiotics in agriculture, owing to their advantages such as high efficiency and safety for human and animal. To obtain genes valuable for further improvement of titer, we have compared two strains（the parental strain and high-yielding strain）by whole genome microarray expression profiling as a discovery platform. In Streptomyces bingchenggensis, there are abundant transporters, which are responsible for transporting various substrates. In the result, some sugar transporter genes showed significant gene expression. 6 time points were designed at 18,48,72,96,144,and 192 hours in two strains. The samples were collected at each time point named W1,W2,W3,W4,W5,W6,H1,H2,H3,H4,H5,and H6.