Gene expression profile of cultured isolated UB in branching (BSN, PTN) and non-branching (HRG) conditions

Genes ontologically classified as proliferative and developmental were down-regulated by HRG, whereas those involved in transport were up-regulated. For example, the mRNA for the GDNF receptors, GFRa1 and ret9 were down-regulated while the mRNA for collecting duct transporters, such as urea transporter2, aquaporin3, sodium-hydrogen exchanger2 were up-regulated in HRG-treated UBs compared with UBs grown in the presence of branch-promoting factors Keywords: Comparing different growth factor treatment Total RNA was extracted from 3 isolated ureteric buds in culture per one treatment condition (1 sample). Three samples (biological replicates) were used for one condition.