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Freeze-thaw pretreatment can improve efficiency of bacterial DNA extraction from meconium

NIAID Data Ecosystem2026-03-13 收录

微生物组DNA提取

新生儿肠道微生物

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP335398 数据链接链接失效反馈

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资源简介：

Measuring bacteria in meconium is an ideal strategy to understand this issue; however, the low efficiency of bacterial DNA extraction from meconium has limited its utilization.

应用场景：

创建时间：

2021-11-30

微生物组DNA提取

微生物群落分析方法比较

Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodolog

NIAID Data Ecosystem160

Evaluation of Methods for the Extraction and Purification of DNA from the Human Microbiome

微生物组DNA提取

微生物群落表征优化

BackgroundDNA extraction is an essential step in all cultivation-independent approaches to characterize microbial diversity, including that associated with the human body. A fundamental challenge in u

NIAID Data Ecosystem70

Table_2_Premature Birth Infants Present Elevated Inflammatory Markers in the Meconium.docx

新生儿肠道微生物

早产生物标志物

Introduction: Prematurity, a well-established risk factor for various intestinal diseases in newborns, results in increased morbidity and mortality. However, the intestinal inflammatory status of pret

NIAID Data Ecosystem50

Comparison of fungal and bacterial genomic DNA extracted by a variety of methods with or without the use of liquid nitrogen, phenol and chloroform.

微生物组DNA提取

DNA纯化

Fusarium oxysporum f. sp. lycopersici (FOL) and Pseudomona syringae pv. syringae (Pss) genomic DNA was isolated from 50 mg of wet fungal and bacterial biomass, respectively.a bModifications as describ

NIAID Data Ecosystem90

Supplementary data: A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communities

微生物组DNA提取

基因组测序优化

Table S1. Mantel correlations in sample-sample distances between each candidate extraction kit and our standardized protocol, for bacterial/archaeal 16S sequence data. Data were raref

DataCite Commons2022-06-17 更新60

Freeze-thaw pretreatment can improve efficiency of bacterial DNA extraction from meconium

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