Comparison of expression profiles for Nicotiana benthamiana agroinfiltrated with caryophyllene synthase and SNARE-RNAi

Here we show for the first time that vesicle fusion is one of multiple pathways in plant cells involved in terpene emissions. We demonstrate a positive feedback on terpene production when vesicle fusion is inhibited in cells producing high levels of terpenes, which relates to an induced proteasome malfunction. Overall design: N. benthamiana leaves were infiltrated with Agrobacterium containing the following expression constructs: (1) pBin+DsRed-RNAi, (2) MtVAMP721e-RNAi+DsRed-RNAi, (3) CST+DsRed-RNAi, (4) CST+MtVAMP721e-RNAi (six plants per construct, one leaf per plant). Infiltrated leaves were harvested seven days post agroinfiltration. Per sample two leaves were pooled, resulting in three biological replicates for each treatment. Followed by deep sequencing, using Illumina Hiseq 2000.