Hari Shroff, Catherine G Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, Eric Betzig (2011) CIL:38598, Homo sapiens, fibroblast. CIL. Dataset

Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green). This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated. The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13.

本数据集展示了人类包皮成纤维细胞表达Dronpa-α-肌动蛋白（红色）和tdEos-肌连蛋白（绿色）的Photoactivation localization microscopy（PALM）图像。此图像揭示，尽管传统衍射极限显微镜表明α-肌动蛋白和肌连蛋白在焦点粘附区共定位，但PALM清晰地显示出这些分子在空间上是分离的。DIC图像对应于CIL 38597的同一图像区域，而衍射极限的TIRF图像则对应于CIL 38599。图像比例尺为2微米。图像由Catherine和James Galbraith提供，并对应于PNASi U S A 2007年12月18日第104卷第51期的第20308-13页图2。