ATAC-seq in con-T or 3D-T cells

The conventional rigid plate-cultured CD8+ T cells (Con-T) or 3D fibrin gel-cultured CD8+ T cells (3D-T) were harvested for ATAC-seq. Then ATAC libraries were generated as the Omni-ATAC protocol which were described as , and Vazyme Tn5 transposase (TD501) was used for transposition. According to manufacturer’s protocol, ATAC-seq library construction using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme). The library was sequenced with an Illumina NovaSeq 6000 (Illumina). The quality of NSG data was assessed by FastQC. ATAC-seq Data were aligned by Bowtie2 to the GenomeReference Consorium human genome (build 38). MACS2 was used to identify peaks of enrichment.