Genetic diversity of the zigzag ladybird beetle, Cheilomenes sexmaculata F. (Coleoptera: Coccinellidae) with its distribution in India and implications for biological control

The zigzag beetle, Cheilomenes sexmaculata, the most important and abundant of all ladybird beetles, feeds on a diverse range of prey. The study was conducted understand the population structure of C. sexmaculata, individuals collected from five different zones, consisting of 25 subpopulations that uniformly representing India. From the ddRAD sequence data we have identified contigs, SNPs, and INDELs were identified for all these 25 subpopulations collected across the India., The adult C. sexmaculata were collected from five different localities belonging to various zones of India viz., Delhi (North), Nagpur (Central), Jorhat (East), Anand (West) and Bengaluru (South) from May to June 2019. The samples were stored in 100% ethanol and frozen at -70°C. Genomic DNA was isolated from each individual separately by grinding in liquid nitrogen, using the method described earlier (Kim et al., 2012). We employed, restriction-site associated DNA sequencing (RADseq) coupled with Illumina sequencing, produces high coverage of homologous SNP (Single Nucleotide Polymorphism) loci. Population genomic analyses from ddRADseq depend on denovoassembly of a set of reference contigs. Raw SNPs/INDELs calls are represented in a single Hap Map file. 
Methods for processing the data: For samples 1-25, 100-1000ng of high-quality genomic DNA was completely digested using Sphl and Mluc1 in a 20-50ul reaction volume (2.4 U per enzyme, New England Biolabs (NEB), Ipswich, MA, USA) and inc..., , # Genetic diversity of the zigzag ladybird beetle, Cheilomenes sexmaculata F. (Coleoptera: Coccinellidae) with its distribution in India and implications for biological control

This readme file was generated on [2024-11-27] by [Narayana Bhat Devate]

GENERAL INFORMATION

Title of Dataset: Data from: Genetic diversity of the zigzag ladybird beetle, Cheilomenes sexmaculata F. (Coleoptera: Coccinellidae) with its distribution in India and implications for biological control

Author/Principal Investigator Information
Name: Dr H S Rakshith
ORCID:
Institution: ICAR-Indian Agricultural Research Institute, New Delhi, India
Address: ICAR-Indian Agricultural Research Institute, New Delhi, India-110012
Email: [[e-mail]](mailto:[e-mail])

Date of data collection: 2019-05-01 to 2019-06-30

Geographic location of data collection: India viz., Delhi (North), Nagpur (Central), Jorhat (East), Anand (West) and Bengaluru (South)

Information about funding sources that support...