Bacterial strains, routine culture conditions, primers, and probes for qPCR and RT-qPCR.

1 TSB, trypticase soy broth; BHI, brain-heart infusion broth; M7H9, Middlebrook 7H9 broth with OADC enrichment; NB, nutrient broth. All cultures were grown under air at 37°C except A. hydrophila, which was grown at 28°C.
2 Pre-rRNA and DNA amplifications used the same primer sets for all organisms except S. aureus, which required the design of an alternative ribosomal DNA-targeted primer set for optimal DNA detection by qPCR.