Plasmodium falciparum intraerythrocytic stage transcription time course for NF54 and isogenic mutant line with altered K13 expression II.

Plasmodium falciparum intraerythrocytic stage transcription four hour time course for NF54 and PB58 (an isogenic line with a piggyBac transposon in the promotor of K13 that alters K13 expression) The parasite strains NF54 and PB58 (the K13 mutant) were maintained in identical standard culture conditions and synchronized by 3 rounds of sorbitol synchronization. A four hour time-course was collected at 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, and 46 hours post invasion (hpi) with triplicate samples at 6, 26 and 38 hpi.