APC from pre-diabetic and Sick NOD mice are less efficient at maintaining Foxp3 expression <i>in vitro</i>.
收藏NIAID Data Ecosystem2026-03-07 收录
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CD4+CD25+ T cells from 12–15 week-old Sick NOD mice were cultured overnight in multiple wells, either alone or with irradiated T-cell depleted spleen cells (APC) from B6, pre-diabetic (11 week-old) NOD or Sick NOD mice and anti-CD3 antibody. Cells were pooled and labeled with anti-CD4, anti-CD25 and anti-Foxp3 antibodies. CD4+CD25+ T cells and APC were pooled from 3 mice. Cells were analyzed for Foxp3 expression by FACS® after gating on CD4+CD25+ cells. Representative results from one of two experiments are shown.
创建时间：
2013-02-21
