Putative pART27-42-derived siRNA duplexes, their 5′ end strength, and the reads of passenger and guide ssRNAs.

1The 5′ end strength (four or two base parings and one 3′ overhang) was calculated following the methods of Freier et al. [44] and Hutvagner [43].2The nucleotides in italic were derived from the loop sequence of pART27-42.3The siRNA duplexes, which followed the 5′ end strength rule (see text for detail) for producing guide and passenger ssRNAs, were in bold (4 bp calculation) or underlined (2 bp calculation).4“Rn” refers to small RNAs which were not among the top 50 small RNAs listed in Table 2.