Analysis of gene expression in brains of DO1 treated and untreated 5xFAD and control mice using a microarray technology

Transcripts of 4 groups of treated and untreated mice (TG+DO1, TG, WT+DO1 and WT) were systematically investigated. Results revealed a clear separation of data obtained from AD and non-AD brains (Figure 6A), confirming previous observations (Landel et al., 2014). Furthermore, we observed that the compound DO1 alters the transcriptional profiles in brains of 5xFAD and wild-type control mice. Group of transgenic (B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V 6799Vas/jax) and wildtype mice were treated with DO1. As control untreated mice were used. 500 ng of total RNA in 11 µl RNase free water was used as starting material for the generation of biotin-labeled cRNA with the Illumina® TotalPrep™ RNA Amplification Kit (ThermoFisher Scientific, Dreieich, Germany), following supplier instructions. cRNA was cleaned up with cRNA filter cartridges before use for subsequent hybridization on Illumina® Sentrix BeadChips (Illumina, San Diego, USA).