Supplementary_Files_Maternal_Methionine_Supplementation.xlsx

Whole-genome bisulfite sequencing (WGBS) was performed on oocytes collected from 16 twin ewe pairs (8 methionine-treated and 8 controls). These ewes were later bred to control rams, and embryos were flushed for WGBS as well. In oocytes,<b> 2,056 differentially methylated cytosines (DMCs)</b> were identified. Additionally, <b>17 mitochondrial DMCs</b><b> </b>were identified, with 12 hypermethylated and 5 hypomethylated. In embryos, <b>113 DMCs </b>were identified. Mitochondrial DNA analysis revealed <b>22 hypermethylated DMCs</b>. To assess the inheritance of methyl marks, we compared DMCs between oocytes and embryos. While no direct overlaps were found in nuclear DNA, 3 CpGs exhibited <b>opposite methylation trends</b>—hypomethylated in oocytes but hypermethylated in embryos. In contrast, 5 <b>mitochondrial DMCs overlapped</b> between oocytes and embryos.