Long Read Transcriptome Sequencing of primary human hepatocytes treated with VPA using PacBio Isoseq

To demonstrate isotools, our LRTS analysis methods, we generated PacBio Iso-Seq data of human hepatocytes treated with the HDAC inhibitor valproic acid, a compound known to induce widespread transcriptional changes. This analysis shows that LRTS provides valuable additional insights for a better understanding of alternative splicing, in particular with respect to complex novel and differential splicing events. IsoTools is made available for the community along with comprehensive documentation at https://github.com/MatthiasLienhard/isotools.