The evolution of facultative symbiosis in corals

We performed single-cell transcriptome analysis of three stony coral species: Oculina patagonica (Opat), Acropora millepora (Amil) and Stylophora pistillata (Spis) Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. Cell hashing was performed using clicktag barcodes in order to overload 10X runs. Instructions for demultiplexing cells are provided.