Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging

Aß peptides derived from the amyloid precursor protein (APP) have been strongly implicated in the pathogenesis of Alzheimer's disease. However, the normal function of APP and the importance of that role in neurodegenerative disease is less clear. We recovered the Drosophila ortholog of APP, Appl, in an unbiased forward genetic screen for neurodegeneration mutants. We performed comprehensive single cell transcriptional and proteomic studies of Appl mutant flies to investigate Appl function in the aging brain. Overall design: Control and Appl deleted fly brains were dissociated, cells were isolated and analyzed using scRNAseq.