Recruitment of RNA Polymerase II by the pioneer transcription factor PHA-4. Caenorhabditis elegans

Using staged embryos, we find many more genes with poised RNA polymerase than had been observed previously in C. elegans embryos, suggesting that early embryos accumulate poised PolII, and that poising is dynamic. Overall design: Embryos were staged at the 8E and Bean stages of development prior to ChIP-seq