Transcriptomic profilling of cisplatin sensitive and resistance in signet ring KATOIII gastric cancer cells. Transcriptomic profilling of cisplatin sensitive and resistance in signet ring KATOIII gastric cancer cells

Purpose: The goal of our study is to identify the differentially expressed genes between cispaltin sensitive and cisplatin resistance gastric cancer cell line. Methods: Transcriptome sequencing of cisplatin sensitive and cisplatin resistance KATOIII cells were generated by Illumina HiSeq TM, for triplicates Results : Using an optimized data analyzed workflow, we mapped 57773 genes and were found 5966 differentially expressed genes between cisplatin sensitive KATOIII and cisplatin resistance KATO/DDP cell lines. Overall design: Acquired cisplatin resistance KATO/DDP cell line was established from parental cell line KATOIII by step wise treated with cispaltin over 6months. KATO/DDP cells were maintained in low dose cisplatin. KATO/DDP cells were allowed to grow in drug free media for at least two passages and transcriptomic sequencing was conducted.