Patient-Derived Tumor Organoid and Fibroblast Assembloid Models for interrogation of the tumor microenvironment in Esophageal Adenocarcinoma

This repository contains original microscopy data from the Sharpe et al. paper, "Patient-Derived Tumor Organoid and Fibroblast Assembloid Models for interrogation of the tumor microenvironment in Esophageal Adenocarcinoma" in Cell Reports Methods 2024.

All whole slide images were obtained using an LM dotSlide slide scanning microscope in Olympus .vsi format and can be opened using the BioFormats library (for example, in QuPath). Wholemount immunofluorescent stains were imaged on a Leica SP8 laser-scanning confocal microscope and are presented as .IMS files, which allows for visualization and further analysis of the 3D data in Imaris (Oxford Instruments).

Data are arranged in subfolders based on the figure they came from (Figures 1-4).