Percentage of fungal taxa amplified in silico.
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One nucleotide mismatch between each target primer and the database sequence was allowed in ecoPCR, except for the two bases at the 3′-end of the primer.*ITS2, used to construct subset 1, was used as a reverse primer in ecoPCR.†ITS5, used to construct subset 2, was used as a forward primer in ecoPCR.‡ITS4, used to construct subset 2, was used as a reverse primer in ecoPCR.§ITS3, used to construct subset 3, was used as a forward primer in ecoPCR.
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2015-12-02
