Phosphorylating activity on glucose and fructose and activities of hexokinase and glucokinase in Y. lipolytica.

Strains were cultured in minimal medium glucose as indicated in Materials and Methods. Extracts and assay of phosphorylating activities were as described in Materials and Methods.a)Hexokinase and glucokinase activities were calculated from the phosphorylation results with glucose and fructose using a Fructose/Glucose phosphorylation ratio of 1.4 for hexokinase ([92], our unpublished results). Figures in brackets indicate the percentage of the corresponding activity in the extract. Results are the mean values ± the standard deviations of four independent cultures.