RNA-sequencing of scrambled control CIN 612 cells and CIN 612 cells depleted of senataxin (SETX) through stable expression of shRNAs

HPV 31 positive cervical keratinocytes (CIN 612 cells) were transduced with lentiviruses to stably express either a scramble control sequence (Scrm CIN 612) or shRNAs targeting the RNA helicase, senataxin (SETX). RNA sequencing was performed on these cell lines to identify cellular genes whose expression was regulated by SETX expression. The experiment was done in biological duplicate, and two different shRNA sequences were used (shSETX1/13 and shSETX2/30).